ABO blood grouping | scientistlaboratory

ABO Blood Grouping - Tile and Tube Method

OBJECTIVE:

To revise the way to grouping the blood by using tile and tube method
 To know the type of sample blood

ABO blood grouping (tile method) - forward grouping

ABO blood grouping (tube method)

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ABO blood grouping (tile method) - reverse grouping

PROCEDURE:
TILE METHOD
1. Place 1 drop of anti-A, 1 drop of anti-B, 1 drop of anti-AB,A cell, B cell, O cell and Anti-D reagent separately on a tile.
2. Add 1 drop of test red cell suspension to each drop of the typing antiserum and 1 drop of serum to each drop of A-cell, B cell and O-cell reagent.
3. Mix the cells and reagent using a clean stick. Spread each mixture evenly on the slide over an area of 10-15 mm diameter.
4. Tilt the slide and leave the test for 2 minutes at room temperature (22°-24°C). Then rock again and look for agglutination.
5. Record the results.

TUBE METHOD
1. Prepare an appropriate 5% of cell suspension in normal saline .Cells being washed in saline for 3 times.
2. Set up 7 clean test tubes for each sample and label them. Add two drops of anti-A in the tube labelled anti-A two drops of anti-B in tube labelled anti-B, two drops of anti-AB in tube labelled anti-AB and two drops of anti-D in tube labelled anti-D
3. As for reverse grouping, add one volume drop of A cells to tube labelled A, one drop of B cells to tube labelled B and one drop of O cells to tube labelled O
4. Add one drop of test cell suspension in each tube.
5. Mix the contents of each tube by centrifuge the tubes at 1000 rpm for one minute.
6. Observe the supernatant fluid for the presence of haemolysis against a well-lighted background.
7. Gently disperse the cell button and check for agglutination against a well-lighted background.
8. Where no agglutination is seen macroscopically examine the contents under microscope.
9. Record the results immediately

RESULT INTERPRETATION:

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